Liu, Q., Luo, Q., Ju, Y. & Song, G. Role of the mechanical microenvironment in cancer development and progression. Cancer Biol. Med. 17, 282–292 (2020).
Google Scholar
Amer, M., Shi, L. & Wolfenson, H. The ‘yin and yang’ of cancer cell growth and mechanosensing. Cancers https://doi.org/10.3390/cancers13194754 (2021).
Lv, J. et al. Cell softness regulates tumorigenicity and stemness of cancer cells. EMBO J. https://doi.org/10.15252/embj.2020106123 (2021).
Fuhs, T. et al. Rigid tumours contain soft cancer cells. Nat. Phys. 18, 1510–1519 (2022).
Google Scholar
Lei, K. et al. Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy. Nat. Biomed. Eng. 5, 1411–1425 (2021).
Google Scholar
Liu, Y. et al. Cell softness prevents cytolytic T-cell killing of tumor-repopulating cells. Cancer Res. 81, 476–488 (2021).
Google Scholar
Tello-Lafoz, M. et al. Cytotoxic lymphocytes target characteristic biophysical vulnerabilities in cancer. Immunity 54, 1037–1054.e1037 (2021).
Google Scholar
Zhou, Y. et al. Cell softness renders cytotoxic T lymphocytes and T leukemic cells resistant to perforin-mediated killing. Nat. Commun. 15, 1405 (2024).
Google Scholar
Dustin, M. L. & Long, E. O. Cytotoxic immunological synapses. Immunol. Rev. 235, 24–34 (2010).
Google Scholar
Finn, O. J. A believer’s overview of cancer immunosurveillance and immunotherapy. J. Immunol. 200, 385–391 (2018).
Google Scholar
Basu, R. et al. Cytotoxic T cells use mechanical force to potentiate target cell killing. Cell 165, 100–110 (2016).
Google Scholar
Basu, R. & Huse, M. Mechanical communication at the immunological synapse. Trends Cell Biol. 27, 241–254 (2017).
Google Scholar
Alibert, C., Goud, B. & Manneville, J. B. Are cancer cells really softer than normal cells? Biol. Cell 109, 167–189 (2017).
Google Scholar
Levental, K. R. et al. Matrix crosslinking forces tumor progression by enhancing integrin signaling. Cell 139, 891–906 (2009).
Google Scholar
Plodinec, M. et al. The nanomechanical signature of breast cancer. Nat. Nanotechnol. 7, 757–765 (2012).
Google Scholar
Tan, Y. et al. Matrix softness regulates plasticity of tumour-repopulating cells via H3K9 demethylation and Sox2 expression. Nat. Commun. 5, 4619 (2014).
Google Scholar
Cappell, K. M. & Kochenderfer, J. N. Long-term outcomes following CAR T cell therapy: what we know so far. Nat. Rev. Clin. Oncol. 20, 359–371 (2023).
Google Scholar
Brown, C. E. et al. Regression of glioblastoma after chimeric antigen receptor T-cell therapy. N. Engl. J. Med. 375, 2561–2569 (2016).
Google Scholar
Sampson, J. H. et al. Immunologic escape after prolonged progression-free survival with epidermal growth factor receptor variant III peptide vaccination in patients with newly diagnosed glioblastoma. J. Clin. Oncol. 28, 4722–4729 (2010).
Google Scholar
Yu, Z., Pestell, T. G., Lisanti, M. P. & Pestell, R. G. Cancer stem cells. Int. J. Biochem. Cell Biol. 44, 2144–2151 (2012).
Google Scholar
Batlle, E. & Clevers, H. Cancer stem cells revisited. Nat. Med. 23, 1124–1134 (2017).
Google Scholar
Masoumi, J. et al. Cancer stem cell-targeted chimeric antigen receptor (CAR)-T cell therapy: challenges and prospects. Acta Pharm. Sin. B 11, 1721–1739 (2021).
Google Scholar
Liu, L. et al. Engineering chimeric antigen receptor T cells for solid tumour therapy. Clin. Transl. Med. 12, e1141 (2022).
Google Scholar
Maude, S. L. et al. Chimeric antigen receptor T cells for sustained remissions in leukemia. N. Engl. J. Med. 371, 1507–1517 (2014).
Google Scholar
Tee, S.-Y., Fu, J., Chen, C. S. & Janmey, P. A. Cell shape and substrate rigidity both regulate cell stiffness. Biophys. J. 100, L25–L27 (2011).
Google Scholar
Tse, J. R. & Engler, A. J. Preparation of hydrogel substrates with tunable mechanical properties. Curr. Protoc. Cell Biol. 10, 10.16 (2010).
Liang, C. et al. Towards an integrative understanding of cancer mechanobiology: calcium, YAP, and microRNA under biophysical forces. Soft Matter 18, 1112–1148 (2022).
Google Scholar
Jiang, Y. et al. Targeting extracellular matrix stiffness and mechanotransducers to improve cancer therapy. J. Hematol. Oncol. https://doi.org/10.1186/s13045-022-01252-0 (2022).
Gilbert, S. M. et al. ATP in the tumour microenvironment drives expression of nfP2X(7), a key mediator of cancer cell survival. Oncogene 38, 194–208 (2019).
Google Scholar
So, C. L. et al. Increased matrix stiffness suppresses ATP-induced sustained Ca2+ influx in MDA-MB-231 breast cancer cells. Cell Calcium 104, 102569 (2022).
Google Scholar
Lembong, J., Sabass, B., Sun, B., Rogers, M. E. & Stone, H. A. Mechanics regulates ATP-stimulated collective calcium response in fibroblast cells. J. R. Soc. Interface 12, 20150140 (2015).
Google Scholar
Furlow, P. W. et al. Mechanosensitive pannexin-1 channels mediate microvascular metastatic cell survival. Nat. Cell Biol. 17, 943–952 (2015).
Google Scholar
Wang, X. et al. Extracellular ATP, as an energy and phosphorylating molecule, induces different types of drug resistances in cancer cells through ATP internalization and intracellular ATP level increase. Oncotarget 8, 87860–87877 (2017).
Google Scholar
Jones, C. A. & Hazlehurst, L. A. Role of calcium homeostasis in modulating EMT in cancer. Biomedicines https://doi.org/10.3390/biomedicines9091200 (2021).
Yoon, C. W. et al. Tumour priming by ultrasound mechanogenetics for CAR T therapy. Nat. Mater. 25, 310–321 (2026).
Google Scholar
Velez, D. O. et al. 3D collagen architecture induces a conserved migratory and transcriptional response linked to vasculogenic mimicry. Nat. Commun. 8, 1651 (2017).
Google Scholar
Fatma, H. & Siddique, H. R. Pluripotency inducing Yamanaka factors: role in stemness and chemoresistance of liver cancer. Expert Rev. Anticancer Ther. 21, 853–864 (2021).
Google Scholar
Fillmore, C. M. & Kuperwasser, C. Human breast cancer cell lines contain stem-like cells that self-renew, give rise to phenotypically diverse progeny and survive chemotherapy. Breast Cancer Res. 10, R25 (2008).
Google Scholar
Zhang, K. et al. IL-24 improves efficacy of CAR-T cell therapy by targeting stemness of tumor cells. Br. J. Cancer 130, 1337–1347 (2024).
Google Scholar
Costa, R. L. B. & Gradishar, W. J. Triple-negative breast cancer: current practice and future directions. J. Oncol. Pract. 13, 301–303 (2017).
Google Scholar
Nelson, R. A., Guye, M. L., Luu, T. & Lai, L. L. Survival outcomes of metaplastic breast cancer patients: results from a US population-based analysis. Ann. Surg. Oncol. 22, 24–31 (2015).
Google Scholar
Hu, J. et al. The mixed subtype has a worse prognosis than other histological subtypes: a retrospective analysis of 217 patients with metaplastic breast cancer. Breast Cancer Res. Treat. 200, 23–36 (2023).
Google Scholar
Berger, C. et al. Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primates. J. Clin. Invest. 118, 294–305 (2008).
Google Scholar
Lele, T. P., Brock, A. & Peyton, S. R. Emerging concepts and tools in cell mechanomemory. Ann. Biomed. Eng. 48, 2103–2112 (2020).
Google Scholar
Kim, J. W. et al. Molecular recording of calcium signals via calcium-dependent proximity labeling. Nat. Chem. Biol. 20, 894–905 (2024).
Google Scholar
O’Neill, B. K. & Laughlin, S. T. Neuronal calcium recording with an engineered TEV protease. ACS Chem. Biol. 13, 1159–1164 (2018).
Google Scholar
Marzagalli, M., Fontana, F., Raimondi, M. & Limonta, P. Cancer stem cells—key players in tumor relapse. Cancers https://doi.org/10.3390/cancers13030376 (2021).
Ghasemi, K. & Ghasemi, K. A brief look at antitumor effects of doxycycline in the treatment of colorectal cancer and combination therapies. Eur. J. Pharmacol. https://doi.org/10.1016/j.ejphar.2021.174593 (2022).
Meumann, N. et al. Hepatocellular carcinoma is a natural target for adeno-associated virus (AAV) 2 vectors. Cancers https://doi.org/10.3390/cancers14020427 (2022).
Ito, M., Takino, N., Nomura, T., Kan, A. & Muramatsu, S. I. Engineered adeno-associated virus 3 vector with reduced reactivity to serum antibodies. Sci. Rep. 11, 9322 (2021).
Google Scholar
Rosenblum, D. et al. CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy. Sci. Adv. https://doi.org/10.1126/sciadv.abc9450 (2020).
Jardin, I. et al. Store-operated calcium entry and its implications in cancer stem cells. Cells https://doi.org/10.3390/cells11081332 (2022).
Wu, Y. et al. Control of the activity of CAR-T cells within tumours via focused ultrasound. Nat. Biomed. Eng. 5, 1336–1347 (2021).
Google Scholar
