Arnold M, Sierra MS, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global patterns and trends in colorectal cancer incidence and mortality. Gut. 2017;66:683–91.
Google ScholarÂ
Aarons CB, Shanmugan S, Bleier JI. Management of malignant colon polyps: current status and controversies. World J Gastroenterol. 2014;20:16178–83.
Google ScholarÂ
Schatzkin A, Freedman LS, Schiffman MH, Dawsey SM. Validation of intermediate end points in cancer research. J Natl Cancer Inst. 1990;82:1746–52.
Google ScholarÂ
Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 1990;61:759–67.
Google ScholarÂ
Peipins LA, Sandler RS. Epidemiology of colorectal adenomas. Epidemiol Rev. 1994;16:273–97.
Google ScholarÂ
Garrett WS. The gut microbiota and colon cancer. Science. 2019;364:1133–5.
Google ScholarÂ
Tilg H, Adolph TE, Gerner RR, Moschen AR. The intestinal microbiota in colorectal cancer. Cancer Cell. 2018;33:954–64.
Google ScholarÂ
Zou S, Fang L, Lee MH. Dysbiosis of gut microbiota in promoting the development of colorectal cancer. Gastroenterol Rep. 2018;6:1–12.
Google ScholarÂ
Castellarin M, Warren RL, Freeman JD, Dreolini L, Krzywinski M, Strauss J, et al. Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma. Genome Res. 2012;22:299–306.
Google ScholarÂ
Kostic AD, Chun E, Meyerson M, Garrett WS. Microbes and inflammation in colorectal cancer. Cancer Immunol Res. 2013;1:150–7.
Google ScholarÂ
Mima K, Nishihara R, Qian ZR, Cao Y, Sukawa Y, Nowak JA, et al. Fusobacterium nucleatum in colorectal carcinoma tissue and patient prognosis. Gut. 2016;65:1973–80.
Google ScholarÂ
Mira-Pascual L, Cabrera-Rubio R, Ocon S, Costales P, Parra A, Suarez A, et al. Microbial mucosal colonic shifts associated with the development of colorectal cancer reveal the presence of different bacterial and archaeal biomarkers. J Gastroenterol. 2015;50:167–79.
Google ScholarÂ
Zouiouich S, Wan Y, Vogtmann E, Porras C, Abnet CC, Shi J, et al. Sample size estimations based on human microbiome temporal stability over 6 months: a shallow shotgun metagenome sequencing analysis. Cancer Epidemiol Biomark Prev. 2025;34:588–97.
Google ScholarÂ
Byrd DA, Chen J, Vogtmann E, Hullings A, Song SJ, Amir A, et al. Reproducibility, stability, and accuracy of microbial profiles by fecal sample collection method in three distinct populations. PLoS ONE. 2019;14:e0224757.
Google ScholarÂ
Byrd DA, Sinha R, Hoffman KL, Chen J, Hua X, Shi J, et al. Comparison of methods to collect fecal samples for microbiome studies using whole-genome shotgun metagenomic sequencing. mSphere. 2020;5:1.
Google ScholarÂ
Gudra D, Shoaie S, Fridmanis D, Klovins J, Wefer H, Silamikelis I, et al. A widely used sampling device in colorectal cancer screening programmes allows for large-scale microbiome studies. Gut. 2019;68:1723–5.
Google ScholarÂ
Rounge TB, Meisal R, Nordby JI, Ambur OH, de Lange T, Hoff G. Evaluating gut microbiota profiles from archived fecal samples. BMC Gastroenterol. 2018;18:171.
Google ScholarÂ
Sinha R, Chen J, Amir A, Vogtmann E, Shi J, Inman KS, et al. Collecting fecal samples for microbiome analyses in epidemiology studies. Cancer Epidemiol Biomark Prev. 2016;25:407–16.
Google ScholarÂ
Vogtmann E, Chen J, Amir A, Shi J, Abnet CC, Nelson H, et al. Comparison of collection methods for fecal samples in microbiome studies. Am J Epidemiol. 2017;185:115–23.
Google ScholarÂ
Vogtmann E, Chen J, Kibriya MG, Chen Y, Islam T, Eunes M, et al. Comparison of fecal collection methods for microbiota studies in Bangladesh. Appl Environ Microbiol. 2017;83:e00361-17.
Zouiouich S, Mariadassou M, Rue O, Vogtmann E, Huybrechts I, Severi G, et al. Comparison of fecal sample collection methods for microbial analysis embedded within colorectal cancer screening programs. Cancer Epidemiol Biomark Prev. 2022;31:305–14.
Google ScholarÂ
Riboli E, Hunt KJ, Slimani N, Ferrari P, Norat T, Fahey M, et al. European Prospective Investigation into Cancer and Nutrition (EPIC): study populations and data collection. Public Health Nutr. 2002;5:1113–24.
Google ScholarÂ
Truong DT, Franzosa EA, Tickle TL, Scholz M, Weingart G, Pasolli E, et al. MetaPhlAn2 for enhanced metagenomic taxonomic profiling. Nat Methods. 2015;12:902–3.
Google ScholarÂ
Truong DT, Tett A, Pasolli E, Huttenhower C, Segata N. Microbial strain-level population structure and genetic diversity from metagenomes. Genome Res. 2017;27:626–38.
Google ScholarÂ
Beghini F, McIver LJ, Blanco-Miguez A, Dubois L, Asnicar F, Maharjan S, et al. Integrating taxonomic, functional, and strain-level profiling of diverse microbial communities with bioBakery 3. eLife. 2021;10:e65088.
McMurdie PJ, Holmes S. phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS ONE. 2013;8:e61217.
Google ScholarÂ
Oksanen J, Blanchet FG, Friendly M, Kindt R, Legendre P, McGlinn D, et al. vegan: community ecology package. R Package Version. 2015;2:2019.
Wirbel J, Pyl PT, Kartal E, Zych K, Kashani A, Milanese A, et al. Meta-analysis of fecal metagenomes reveals global microbial signatures that are specific for colorectal cancer. Nat Med. 2019;25:679–89.
Google ScholarÂ
Thomas AM, Manghi P, Asnicar F, Pasolli E, Armanini F, Zolfo M, et al. Metagenomic analysis of colorectal cancer datasets identifies cross-cohort microbial diagnostic signatures and a link with choline degradation. Nat Med. 2019;25:667–78.
Google ScholarÂ
Zhao N, Chen J, Carroll IM, Ringel-Kulka T, Epstein MP, Zhou H, et al. Testing in microbiome-profiling studies with MiRKAT, the microbiome regression-based kernel association test. Am J Hum Genet. 2015;96:797–807.
Google ScholarÂ
Aguado Loi CX, Martinez Tyson D, Chavarria EA, Gutierrez L, Klasko L, Davis S, et al. Simple and easy:’ providers’ and latinos’ perceptions of the fecal immunochemical test (FIT) for colorectal cancer screening. Ethn Health. 2020;25:206–21.
Google ScholarÂ
Gwede CK, Sutton SK, Chavarria EA, Gutierrez L, Abdulla R, Christy SM, et al. A culturally and linguistically salient pilot intervention to promote colorectal cancer screening among Latinos receiving care in a Federally Qualified Health Center. Health Educ Res. 2019;34:310–20.
Google ScholarÂ
Davis SN, Govindaraju S, Jackson B, Williams KR, Christy SM, Vadaparampil ST, et al. Recruitment techniques and strategies in a community-based colorectal cancer screening study of men and women of african ancestry. Nurs Res. 2018;67:212–21.
Google ScholarÂ
Davis SN, Christy SM, Chavarria EA, Abdulla R, Sutton SK, Schmidt AR, et al. A randomized controlled trial of a multicomponent, targeted, low-literacy educational intervention compared with a nontargeted intervention to boost colorectal cancer screening with fecal immunochemical testing in community clinics. Cancer. 2017;123:1390–400.
Google ScholarÂ
Baxter NT, Koumpouras CC, Rogers MA, MTt Ruffin, Schloss PD. DNA from fecal immunochemical test can replace stool for detection of colonic lesions using a microbiota-based model. Microbiome. 2016;4:59.
Google ScholarÂ
Hale VL, Chen J, Johnson S, Harrington SC, Yab TC, Smyrk TC, et al. Shifts in the fecal microbiota associated with adenomatous polyps. Cancer Epidemiol Biomark Prev. 2017;26:85–94.
Google ScholarÂ
Zhang Y, Yu X, Yu E, Wang N, Cai Q, Shuai Q, et al. Changes in gut microbiota and plasma inflammatory factors across the stages of colorectal tumorigenesis: a case-control study. BMC Microbiol. 2018;18:92.
Google ScholarÂ
Bateman LA, Ku WM, Heslin MJ, Contreras CM, Skibola CF, Nomura DK. Argininosuccinate Synthase 1 is a Metabolic Regulator of Colorectal Cancer Pathogenicity. ACS Chem Biol. 2017;12:905–11.
Google ScholarÂ
Young C, Wood HM, Fuentes Balaguer A, Bottomley D, Gallop N, Wilkinson L, et al. Microbiome analysis of more than 2000 NHS bowel cancer screening programme samples shows the potential to improve screening accuracy. Clin Cancer Res. 2021;27:2246–54.
Google ScholarÂ
Young C, Wood HM, Seshadri RA, Van Nang P, Vaccaro C, Melendez LC, et al. The colorectal cancer-associated faecal microbiome of developing countries resembles that of developed countries. Genome Med. 2021;13:27.
Google ScholarÂ
Grobbee EJ, Lam SY, Fuhler GM, Blakaj B, Konstantinov SR, Bruno MJ, et al. First steps towards combining faecal immunochemical testing with the gut microbiome in colorectal cancer screening. U Eur Gastroenterol J. 2020;8:293–302.
Google ScholarÂ

